目的： 研究新城疫病毒（Newcastle disease virus，NDV）弱毒力株HBNU/LSRC/F3对人食管癌ECA109细胞凋亡的影响，并与其他两株NDV（弱毒力株LaSota株、中等毒力Mukteswar株）的抗肿瘤作用进行比较。 方法： 体外培养ECA109细胞，不同NDV经扩增后分别感染ECA109细胞，CCK-8法检测各NDV对ECA109细胞增殖的抑制作用，激光共聚焦显微镜观察经各NDV感染后ECA109细胞的凋亡情况，流式细胞术检测其早期细胞凋亡，DNA琼脂糖凝胶电泳检测其晚期凋亡情况。 结果： HBNU/LSRC/F3株NDV能抑制ECA109细胞的增殖，且强于LaSota株，但稍弱于Mukteswar株（P<0.05）。ECA109细胞感染5×10-4Hu/ml NDV时，HBNU/LSRC/F3株、Mukteswar株、LaSota株对ECA109细胞的增殖抑制率分别为（31.43±157）%、（39.87±1.99）%和（19.89±0.99）%。流式细胞仪检测结果显示，HBNU/LSRC/F3株感染后ECA109细胞的早期凋亡率为（21.32±0.44）%，而Mukteswar株和LaSota株的早期凋亡率为（22.27±0.23）%和（14.32±0.61）%；激光扫描共聚焦显微镜和DNA琼脂糖凝胶电泳检测结果显示，Mukteswar株以诱导晚期凋亡为主，HBNU/LSRC/F3株以诱导ECA109细胞早期凋亡为主，且较LaSota株明显。 结论： 弱毒力HBNU/LSRC/F3株可有效抑制ECA109细胞增殖，诱导ECA109细胞早期凋亡，虽略低于中等毒力Mukteswar株，但远高于弱毒力LaSota株，因此HBNU/LSRC/F3株可能具有更高的抗肿瘤临床应用价值。

Objective:To explore the impact of Newcastle disease virus (lentogenic HBNU/LSRC/F3) strain on apoptosis of esophageal cancer ECA109 cells, and to compare with the other two strains (lentogenic LaSota strain and mesogenic Mukteswar strain) of their anti-tumor effects. Methods: ECA109 cells were cultured in vitro and infected by three NDV strains respectively. The proliferation inhibiting effects of NDVs on ECA109 cells were detected by CCK-8. The apoptosis of ECA109 cells after NDV infection was detected by laser confocal microscopy. Flow cytometry and agarose gel electrophoresis were used to detect the early and late apoptosis of ECA109 cells, respectively. Results: HBNU/LSRC/F3 NDV strain suppressed the proliferation of ECA109 cells, which was stronger than LaSota strain and weaker than Mukteswar strain (P<0.05). The proliferation inhibition rates of HBNU/LSRC/F3 strain, Mukteswar strain and LaSota strains on ECA109 cells were (39.87±1.99)%, (31.43±1.57)% and (19.89±0.99)%, respectively, infected by 5×10-4 Hu/ml NDV. Flow cytometry showed that the early apoptosis rate of ECA109 cells infected by HBNU/LSRC/F3 strain was (21.32±0.44)%, while Mukteswar strain was (22.27±0.23)% and LaSota strain was (14.32±0.61)%. Laser scanning confocal microscopy and DNA gel electrophoresis showed that Mukteswar strain primarily induced the late apoptosis. Whereas, HBNU/LSRC/F3 strain primarily induced the early apoptosis, which was more obvious than LaSota strain. Conclusion: Lentogenic HBNU/LSRC/F3 strain can effectively inhibit ECA109 cell proliferation and induce early apoptosis of ECA109 cells, which was lower than Mukteswar strain and significantly higher than LaSota strain. Therefore, HBNU/LSRC/F3 strain may have a high clinical value for anti-tumor therapy.

河北省自然科学基金资助项目（No. C2010001450）